首页> 外文OA文献 >Proteomic analysis of plasma exosomes to differentiate malignant from benign pulmonary nodules
【2h】

Proteomic analysis of plasma exosomes to differentiate malignant from benign pulmonary nodules

机译:血浆外泌体蛋白质组学分析,以区分恶性肺结核

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Abstract Background It is difficult to distinguish benign pulmonary nodules (PNs) from malignant PNs by conventional examination. Therefore, novel biomarkers that can identify the nature of PNs are needed. Exosomes have recently been identified as an attractive alternative approach since tumor-specific molecules can be found in exosomes isolated from biological fluids. Methods Plasma exosomes were extracted via the exoEasy reagent method. The major proteins from plasma exosomes in patients with PNs were identified via labelfree analysis and screened for differentially expressed proteins. A GO classification analysis and KEGG pathway analysis were performed on plasma exosomal protein from patients with benign and malignant PNs. Results Western blot confirmed that protein expression of CD63 and CD9 could be detected in the exosome extract. Via a search of the human Uniprot database, 736 plasma exosome proteins from patients with PNs were detected using high-confidence peptides. There were 33 differentially expressed proteins in the benign and malignant PNs. Of these, 12 proteins were only expressed in the benign PNs group, while 9 proteins were only expressed in the malignant PNs group. We further obtained important information on signaling pathways and nodal proteins related to differential benign and malignant PNs via bioinformatic analysis methods such as GO, KEGG, and String. Conclusions This study provides a new perspective on the identification of novel detection strategies for benign and malignant PNs. We hope our findings can provide clues for the identification of benign and malignant PNs.
机译:摘要背景难以通过常规检查将良性肺结核(PNS)与恶性PNS区分开来。因此,需要识别PNS性质的新型生物标志物。最近已经鉴定为具有吸引力的替代方法,因为肿瘤特异性分子可以在从生物液中分离的外来。方法通过exoeasy试剂法萃取等离子体外泌体。通过Labelfree分析鉴定PNS患者血浆外来血浆的主要蛋白质,并筛选差异表达蛋白质。对来自良性和恶性PNS患者的血浆外泌体蛋白进行GO分类分析和KEGG途径分析。结果Western印迹确认可以在外出提取物中检测CD63和CD9的蛋白质表达。通过搜索人的UNIPROT数据库,使用高置信肽检测来自PNS患者的736个血浆外来蛋白。良性和恶性PNS中有33种差异表达的蛋白质。其中,12个蛋白质仅在良性PNS组中表达,而9个蛋白质仅在恶性PNS组中表达。我们进一步通过生物信息分析方法进一步获得了与差异良性和恶性PNS相关的信号传导途径和节点蛋白的重要信息,例如GO,Kegg和String。结论本研究提供了一种关于鉴定良性和恶性PNS的新型检测策略的新视角。我们希望我们的研究结果可以提供识别良性和恶性PNS的线索。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号