Over Production of Lignocellulosic Enzymes of Coriolus Versicolor by GeneticEngineering Methodology

摘要

The project seeks to understand the biological and chemical processes involved inthe secretion of the enzyme polyphenol oxidase (PPO) by the hyphae, the basic unit of the filamentous fungus Coriolus versicolor. These studies are made to determine rational strategies for enhanced secretion of PPO, both with the use of recombinant DNA techniques (Howard University), and without (Clark-Atlanta). This effort is done in concert with work at Clark-Atlanta University but focuses on recombinant DNA techniques to enhance enzyme production. The major thrust of this project was two-fold: (1) to mass produce C. versicolor tyrosinase (polyphenol oxidase) by genetic engineering as well as cultural manipulations; (2) to utilize polyphenol oxidase (PPO) as a biocatalyst in the processing of lignocellulose as a renewable energy resource. Wood-decay within forests, a significant renewable photosynthetic energy resource, is caused primarily by Basidiomycetous fungi, i.e., white-rot fungi. In the case of the resource, is caused primarily by Basidiomycetous fungi, i.e., white-rot fungi. In the case of the white-rot fungus, C. versicolor, its degradative capacity results from the ability to elaborate extracellular cellulolytic and ligninolytic enzymes.