Viral Vectors and Methods for Producing and Using the Same.

摘要

A recombinant hybrid virus, including: (a) a deleted adenovirus vector genome comprising the adenovirus 5 feet and 3 feet cis-elements for viral replication and encapsidation, and further comprising a deletion in an adenovirus genomic region selected from the group consisting of: (1) the polymerase region, wherein said deletion essentially prevents the expression of a functional polymerase protein from said deleted region and said hybrid virus does not otherwise express a functional polymerase protein, (2) the preterminal protein region, wherein said deletion essentially prevents the expression of a functional preterminal protein from said deleted region, and said hybrid virus does not otherwise express a functional preterminal protein, and (3) both the regions of (1) and (2); and (b) a recombinant adeno-associated virus (AAV) vector genome flanked by the adenovirus vector genome sequences of (a), said recombinant AAV vector genome comprising (1) AAV 5 feet and 3 feet inverted terminal repeats, (2) an AAV packaging sequence, and (3) a heterologous nucleic acid sequence, wherein said heterologous nucleic acid sequence is flanked by the 5 feet and the 3 feet AAV inverted terminal repeats of (1).