Electrodeposition Leading to Immobilized Biosensor Electrodes.

摘要

Enzymes attached to colloidal gold were successfully electrodeposited on several types of conducting materials, including carbon rods, gold/glass electrodes, and platinum gauze electrodes. Two enzymes, xanthine oxidase and glucose oxidase, after adsorption to colloidal gold showed good activity in solution prior to electrodeposition and retained this catalytic activity after electrodeposition. One of the electrodeposited enzymes, glucose oxidase, exhibited electron transfer activity when an electron transfer agent was added to the electrode buffer. These results showed the feasibility of using glucose oxidase to determine glucose concentrations up to 20 mM by amperometric measurements. These results should be applicable to the development of other electroactive enzymes for biosensor applications.