Immobilization and Testing of Proteins on Microsensors

摘要

Proteins were covalently immobilized onto surfaces using various techniques,including chemical coupling using glutaraldehyde or via biotin-streptavidin. Testing of a receptor-based biosensor system was accomplished using the 1,4-Dihydropyridine receptor. Antibody-based testing included detection of botulinum toxin and Mojave toxin, Hybridized DNA was used to determine the feasibility of detection of bacteria using gene probes. Antibody and DNA detection were tested on fiber optic (evanescent wave sensor) and potentiometric (light addressable poteniometric sensor) microsensors. The microsensor testing indicated that detection of toxins and DNA was rapid, sensitive, and selective.