Assessing the Feasibility of an In Vitro Cytotoxicity Method to Detect Harmful Ubiquitous Chemicals

摘要

The objective of this work was to assess the feasibility of accomplishing aqueous extracts of soil samples and determining if the extracted solution induced adverse effects in the human myelomonocytic cell line, HL60. Dosing of HL60 cells was accomplished over a 24hour period using 100% of extracted media from standard soil samples containing known contaminants. Assessments of viability, apoptosis, reduced thiols, and mitochondrial membrane integrity were accomplished by argon-ion laser flow cytometric analysis, using chemical labels specific for each endpoint. The in vitro cytotoxicity data was compared with the results of Microtox and Mutatox tests was well as earthworm and plant toxicity tests. In vitro cytotoxicity tests results exhibited good correlation with other tests results.