Outcome Based Screening for Prognostic Phospho-RTK (Receptor Tyrosine Kinase) Antibodies Using Tissue Microarrays

摘要

Receptor Tyrosine Kinases (RTKs) have been identified as potential targets for both breast cancer prognosis and therapy. We proposed use of tissue microarrays to evaluate the prognostic value of RTKs with emphasis on the phosphorylation status of these receptors. Analysis of a series of phospho- receptor antibodies on large cohorts on tissue microarrays should reveal which RTKs are most likely to be of prognostic and therapeutic value. As of this progress report, we have completed construction of the tissue microarrays and completed collection of the clinical data. We have also completed and submitted a pilot study of RTKs using conventional analysis of this array. In the proposal we show automated analysis of the arrays has potential to reveal relationships that are undetectable by conventional methods. We have now completed our efforts in construction of the device capable of high through-put automated array analysis. We have completed studies using this technology (called AQUA, for automated quantitative analysis)on a number of RTKs. Although we were unable to successfully use phosphor-An, the quantitative assess of the RTKs themselves provided novel information with respect.