Application of Water-Insoluble Complexes of Klininogenases, Inhibitors and Kininases to Kinin Research

摘要

A variety of methods have been available for coupling proteins with water-insoluble carriers. In one previous study trypsin was coupled to the copolymer of maleic anhydride and ethylene (EMA) using hexamethylenediamine as cross-linking agent. The proposed mechanism of action involves the binding of the enzyme covalently via the epsilon-amino group of lysine in the protein. Because many of the kininogenases and kininases are labile enzymes badly in need of stabilization, the authors started to study the feasibility of binding some of these enzymes and their inhibitors to water-insoluble carriers.