Cryopreservation of Red Cells: Freeze-Preservation of Non-Rejuvenated Red Cells, Indated-Rejuvenated Red Cells, and Outdated-Rejuvenated Red Cells.

摘要

Preserved red cells are transused to increase the delivery of oxygen to tissue. It is also essential that the preserved red cells circulate in order increase the red cell mass and to improve the oxygen carrying capacity. The delivery of oxygen to tissue immediately after transfusion of preserved red cells depends to a great extent on their affinity for oxygen. Red cells stored in ACD for 7 days at 4 C maintain their ability to carry oxygen, but their ability to release it is impaired. It was not untill 1967 that the correlation between oxygen transport function and the red cell 2,3 DPG level was appreciated. More recently, investigators have realized the importance of the oxygen delivering capacity of transfused red cells during the first 4 hours after transfusion. Red cells that have low 2,3 DPG levels and increased affinity for oxygen will increase the cardiac output and/or decrease the venous PO2 for 4 hours after transfusion. CPD-preserved red cells are more likely to have normal oxygen transport than are ACD-preserved red cells. During storage at 4 C the oxygen transport function of CPD red cells is maintained by purine nucleoside supplementation, and during freeze-preservation it is maintained with glycerol.