Efficacy of Gamma-glutamylcysteine (GGC) in Ischemia-reperfusion Injury.

摘要

Experiments were performed to study Gamma-glutamycysteine (GGC) a precursor dipeptide for glutathione (GSH) for its ability to inhibit oxidative stress in human umbilical vein endothelial cells (HUVEC). HUVEC were used as a model to assess cellular biochemical changes that occur in hemorrhagic shock. We found that GGC protects against oxidative stress by alteration of gene expression of antioxidant defense independent of increased induction of endogenous GSH synthesis. In mixture studies looking at the effects of GGC with conjugated linoleic acid in HUVEC, we found that the mixture protected against oxidative stress similar to treatment with GGC alone. Therefore, GGC is likely a unique compound that exerts protection from oxidative stress by novel by modulation of gene expression of antioxidant defense enzymes. We have also develop a sensitive high performance liquid chromatographic method (HPLC) using fluorimetric detection to measure cellular changes in GGC.