Structure and Functional Studies on Dengue-2 Virus Genome

摘要

The overall objective of this project is to analyze the structure of Dengue-2 virus RNA using recombinant DNA technology in order to understand the biological role of the specific antigens coded by the RNA genome after infection of the host cell. The proposed project is to be carried out in the following steps. 1) Synthesize the cDNA copy of the viral RNA using reverse transcriptase; 2) Clone the cDNA using E.coli host/vector system in order to get large amounts of cDNA; 3) Physically map these cDNA clones using several restriction endonuclease; 4) Location of the genes for the various viral antigens on the cDNAs is to be identified; 5) DNA sequence analysis of cDNA coding for specific viral genes is to be carried out; 6) Finally, the gene for V3 (the glycoprotein E) is to be expressed in E.coli. Keywords: Clones, Gene mapping, Enzymes. (kt)