Genetic Analysis of the Presentation of Minor Lymphocyte Stimulating Determinants. I. Combined Importance of MHC and non-MHC Influences

摘要

In the course of studying the MHC restriction of minor lymphocyte stimulating (Mls) determinants, we observed that variation in the ability to present Mls(c) determinants occurred with stimulator cells from different mouse strains that express the same class II MHC restricting elements; for example, one Ia(d)-bearing strain, C3H.HTG, presented this non-MHC moiety, whereas another, C3H.OH, could not. As another example, the prototype Mls(b) nonstimulatory H-2(d) stimulator cell BALB/c, was shown to encode Mls(c) even though it failed to trigger proliferation across this non-MHC barrier. In contrast H-2(d)-compatible DBA/2 stimulator cells were capable of eliciting detectable levels of unprimed responder T cell proliferation across an Mls(c) difference. Even with the BALB/c H-2(d) haplotype, these BALB.K stimulator cells presented Mls(c) (but not MHC) less effectively than H-2(k)-compatible C3H/HeJ stimulator cells. Analysis of the Mls(c)-presenting capacity of stimulator cells obtained from (BALB.K x C3H) F2 x BALB.K first backcross and (BALB.K x C3H)F2 animals indicated than non-MHC-control influencing stimulatory ability of this non-H2 Ag was multigenic. In addition, the capacity of DBA/2 to present Mls(a) determinants more effectively than MHC-identical LT/ChReSv stimulator cells may indicate that the presentation of this Mls specificity is also influenced by non-MHC Ir genes. Reprints. (AW)