首页> 美国卫生研究院文献>Immunology >Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo.
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Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo.

机译:绵羊淋巴细胞单克隆抗体。 I.体内抗原刺激后淋巴组织上的MHC II类分子的鉴定以及淋巴传播细胞上II类表达水平的变化。

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摘要

A rat monoclonal antibody is described that recognizes sheep MHC Class II antigen and appears to recognize a determinant that is non-polymorphic. This antibody precipitates polypeptides of apparent molecular weight 33,000 and 28,000/29,000 (under non-reducing conditions). The binding of this antibody was inhibited by an anti-human DP, DQ, DR beta-chain monoclonal (DA6.231) and its reactivity on murine cells suggests specificity for the I-E gene product. Using this antibody we have studied the distribution of Class II molecules in sheep and also the variations in Class II expression by cells in both peripheral and efferent lymph, produced as a result of in vivo antigen inoculation.
机译:描述了一种鼠单克隆抗体,它识别绵羊MHC II类抗原,并且似乎识别非多态性的决定簇。该抗体沉淀表观分子量为33,000和28,000 / 29,000(在非还原条件下)的多肽。该抗体的结合被抗人DP,DQ,DRβ链单克隆抗体(DA6.231)抑制,并且其对鼠细胞的反应性提示了对I-E基因产物的特异性。使用该抗体,我们研究了羊中II类分子的分布,以及由于体内抗原接种而产生的外周和传出淋巴中的细胞II类表达的变化。

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