首页> 外文期刊>Physiological Research >Effects of Adenosine A(1) Receptor Antagonism on Lipogenesis and Lipolysis in Isolated Rat Adipocytes
【24h】

Effects of Adenosine A(1) Receptor Antagonism on Lipogenesis and Lipolysis in Isolated Rat Adipocytes

机译:腺苷A(1)受体拮抗作用对离体大鼠脂肪细胞脂肪生成和脂解的影响

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

Adenosine is secreted from adipocytes, binds to adenosine A(1) receptor and modulates various functions of these cells. In the present study, the effects of an adenosine A(1) receptor antagonist ( DPCPX; 0.01, 0.1 and 1 mu M) on lipogenesis, glucose transport, lipolysis and the antilipolytic action of insulin were tested in rat adipocytes. DPCPX had a very weak effect on lipogenesis and did not significantly affect glucose uptake. In adipocytes incubated with 1 mu M DPCPX, lipolysis increased. This effect was blunted by insulin and by a direct inhibitor of protein kinase A. Moreover, 0.1 mu M DPCPX substantially enhanced the lipolytic response to epinephrine and increased cAMP in adipocytes. However, DPCPX was ineffective when lipolysis was stimulated by direct activation of protein kinase A. Adipocyte exposure to epinephrine and insulin with or without 0.1 mu M DPCPX demonstrated that this antagonist increased the release of glycerol. However, despite the presence of DPCPX, insulin was able to reduce lipolysis. It is concluded that DPCPX had a weak effect on lipogenesis, whereas lipolysis was significantly affected. The partial antagonism of adenosine A(1) receptor increased lipolysis in cells incubated with epinephrine alone and epinephrine with insulin due to the synergistic action of 0.1 mu M DPCPX and epinephrine.
机译:腺苷从脂肪细胞中分泌出来,与腺苷A(1)受体结合并调节这些细胞的各种功能。在本研究中,在大鼠脂肪细胞中测试了腺苷A(1)受体拮抗剂(DPCPX; 0.01、0.1和1μM)对脂肪生成,葡萄糖转运,脂解和胰岛素的抗脂解作用的影响。 DPCPX对脂肪生成的作用非常弱,并且不会显着影响葡萄糖的摄取。在用1μMDPCPX孵育的脂肪细胞中,脂肪分解增加。胰岛素和蛋白激酶A的直接抑制剂使这种作用减弱。此外,0.1μM DPCPX显着增强了对肾上腺素的脂解反应,并增加了脂肪细胞中的cAMP。但是,当直接激活蛋白激酶A刺激脂解时,DPCPX无效。有或没有0.1μMDPCPX的脂肪细胞暴露于肾上腺素和胰岛素,证明该拮抗剂增加了甘油的释放。然而,尽管存在DPCPX,胰岛素仍能够减少脂肪分解。结论是,DPCPX对脂肪生成的作用较弱,而脂解作用则受到显着影响。腺苷A(1)受体的部分拮抗作用增加了单独使用肾上腺素和肾上腺素与胰岛素孵育的细胞的脂解作用,这是由于0.1μM DPCPX和肾上腺素的协同作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号