Dietary Fiber Content of Cross-linked Phosphorylated Resistant Starch (RS4) Determined by the Prosky and McCleary MethodsPart I. Factors Affecting In Vitro Digestion of Starch in a Food Sample1

摘要

Porcine pancreatic a-amylase and Aspergillus niger glucoamy-lase are used often in dietary fiber assay of foods. In this article (part I) we review important variables that influence the removal of digestible starch from a food sample by these two enzymes. A second companion article (part II) provides a comparison of the starch digestion conditions of the Prosky versus McCleary methods, assay data for resistant starch (RS) samples, and a hypothesis for why the McCleary method produces a relatively low recovery of dietary fiber from cross-linked phosphorylated RS4 (17). The McCleary method is detailed in AACCI Approved Methods 32-45.01 and 32-50.01 (1), which are equivalent to AOAC Methods 2009.01 and 2011.25 (2). The Prosky method is