Production of L-phenyl Acetyl Carbinol (L-PAC) by Different Novel Strains of Yeasts in Molasses and Sugar Cane Juice as Production Medium

摘要

Isolation and screening of novel strains of yeasts from natural sources like fruits and sugarcane juice for biotransformation studies was investigated. These strains were identified at the Institute of Microbial Technology, Chandigarh, basing on sequencing of D_1/D_2 domain of 26S rRNA gene and assigned MTCC numbers. These three strains were designated as Candida pseudointermedia MTCC No. 6225 (BGY), Issatchenkia orientials MTCC No. 6351 (DY), Candida pseudointermedia MTCC No. 6352 (SCY), respectively. Production of L-phenyl acetyl carbinol (L-PAC) through biotransformation of benzaldehyde by free cells of the yeast of different strains has been attempted. In our experiments the L-PAC product obtained from benzaldehyde through biotransformation by four different yeasts like baker's yeast, Candida pseudointermedia (6225), Candida pseudointermedia (6352) and Issatchenkia orientials (6351).