The production of living biomass of bacteria of the genera lactobacillos, Bifidobacterium and Streptococcus with probiotic activity in culture medium on the basis of molasses and sugar cane juice

摘要

"Production of living biomass of bacteria of the genera lactobacillus,Bifidobacterium, streptococcus with probiotic activity in culture medium on the basis of molasses and broth of sugar cane "formed by 7% molasses of sugar cane, or 7% of juice of sugar cane and D.And 2,7% of yeast extract, with the strains Lactobacillus acidophilus ATCC 314; Streptococcus thermophilus ATCC 19258,Bifidobacterium longum ATCC 15707; Lactobacillus casei ATCC 25180; Bifidobacterium bifidumatcc1 1863; Bifidobacterium infantis ATCC 15697; Lactobacillus plantarum ATCC 8014; Lactobacillus delbrueckiiSubsp. Bulgaricus ATCC 7994 and Lactobacillus fermentum lpb1 isolated from intestinal microflora of hillbilly chicken.The present invention involves the preparation of medium, the optimization of the most influential factors in the process, including composition of the substrate (molasses of sugar cane.Juice of cane sugar and yeast extract) is physical variables (incubation temperature, initial pH of medium, time of fermentation).The main goal of optimization is to provide lower costs and higher incomes in the production of probiotic cells (living biomass) produced in laboratory scale and industrial.Finally, the present invention involves freeze drying of cells as a way to preserve and increase the period of viability of probiotic cells.