Isolation and Culture Conditions of a Pseudomonas Strain Capable of Removing NH_4~+ and NO_3~- Simultaneously in Anaerobic Conditions

摘要

A bacterial strain AE-1-3, isolated from soil and wastewater identified as Pseudomonas strain, removed NH_4~+ and NO_3~- simultaneously in anaerobic cultivation in a medium containing 0.1% NH4NO3 and 3.0% glucose. The strain removed NH_4~+, NO and NCVcompletely in 15 days of anaerobic cultivation. Though NO_3~- removed completely, 33% of NH_4~+ remained in 15 day of incubation in 1 % glucose and 0.1% NH_4NO_3 medium. The bacterium could remove 0.1% NH_4NO_3 completely in a short time by addition ofCu~(2+), Zn~(2+), Sn~(2+) in 0.5% glucose medium. By chaning the metal concentration, 0.3% NH_4NO_3 could be removed completely.