Biological activities of polysaccharide extracted from the fruit body and cultured mycelia of Phellinus linteus IY001

摘要

This study was conducted to investigate the biological activities of polysaccharide extracted from the fruit body and cultured mycelia of Phellinus linteus IY001. All fractions were extracted by hot water, in the next, Fr. I, Fr. II, Fr. III and Fr. IV were polysaccharide obtained by ethanol precipitation or ultrafiltration. The highest antitumor activity against sarcoma 180 in ICR mice was observed in Fr. III and Fr. IV at the level 85 percent, but the antitumor activity had no connection with their anticomplementary activity in vitro, it might probably be due to extraction of hot water. All fractions promoted the production of nitric oxide and TNF-#alpha# in macrophage, addition of Fr. I and Fr. II resulted in production of nitric oxide(35.9-37.6 #mu#M) and of the TNF-#alpha# production(8,696.2-9,420pg/ml). All fractions inhibited the lipid peroxidation induced by AsA/Fe~(2+), ADP/NADPH/Fe~(3+) and CCl_4/NADPH in rat liver microsomes, and Fr. III showed the electron donating ability stronger than tocopherol in assay system using DPPH. From these results, it is suggested that all fractions contain immunoregulatory components which may protect cellular materials from the oxidative damages by their radical scavenging activities.