Study on immune-stimulating activity of beta-glucan isolated from the cell wall of yeast mutant Saccharomyces cerevisiae IS2

摘要

Yeast cell wall mutant, Saccharomyces cerevisiae IS2 was screened by the NTG treatment of Saccharomyces cerevisiae KCTC 7911. The mutant was highly resistant to zymolase, which specifically degrades beta-1,3-D-gIucose chain of beta-glucan and mechanical disruption by glass beads. These phenomena demonstrate that the yeast mutant has cell wall structure different from the wild-type. The beta-glucan of yeast mutant and wild-type strains was recovered by sequential extraction with NaOH. The injectionof beta-glucan into the abdominal cavity of mouse resulted in an increase in the number of peritoneal immune cells, NO (nitric oxide) production, and phagocytic activity of macrophage. The numher of immune cells was found to be 3.90 X 10~6 cells/10 mL and 5.48 X 10~6 cells/ 10 ml, with the wild-type and mutant beta-glucan, respectively. The effect on the NO production and phagocytic activity of mutant beta-glucan were 1.69 and 1.43-fold higher than those of wild-type. These results indicate that the immuno-stimiilating activity of alternated beta-glucan from mutant yeast is higher than that of wild-type.