Antioxidant and Neuronal Cell Protective Effects of Methanol Extract from Schizandra chinensis using an in vitro System

摘要

In this study, the antioxidant and neuronal cell protective effects of methanol extract from Schizandra chinensis were evaluated. The proximate composition and total phenolics content of the extract were as follows. 64.88% nitrogen free extract, 10.56% crude fiber, 10.22% moisture, 8.33% crude protein, 5.05% ash, 0.96% crude fat, and 83.05 mg/g of total phenolics. In assays the methanol extract of Schizandra chinensis presented feme reducing/antioxidant power (RAP) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity in a dose-dependent manner. In a cell viability assay using 3-(4,5-dimethyltrnazol-2-yl)-2,5-diphenyRetrazoliumbromide (MTT), the methanol extract showed protective effect against H_2O_2-iriducedneurotoxicity, and lactate dehydrogenase (LDH) release into medium was also inhibited by various concentrations of extracts (68-80%). Cell viability after treatment of the methanol extract was higher than that shown for vitamin C (100 mu M) using a neutral red uptake (NRU) assay. Therefore, these date suggest that the methanol extract of Schizandra chinensis may be useful for neurodegenerative diseases including Alzheimer s disease.