Study of the Protective Effect of Schizandra Chinensis Polysaccharide on Oxidative Damage of Mouse Spermatozoa in Vitro

摘要

The aim of the study: This study was conducted to evaluate oxidative stress damage model caused by reactive oxygen species production was prepared by heating injury and protective effect of SCP on spermatozoa membrane structure and function in vitro. Materials and methods: mouse eupyrene sperm suspensions were prepared and were randomly divided into six groups. The control group was maintained with PBS. The positive medicine group was received PBS contained vitamin E (0.25 mg/ml). The SCP group was then given SCP (0.2, 0.4 and 0.8 mg/ml) respectively. All the groups except control group were treated with using heat stress injury. Degree of injury of spermatozoa membrane lipid peroxidation was detected. Results: The vitality of total superoxide dismutase (T-SOD) was increased and the quality of malondialdehyde (MDA) was decreased in different degree with each concentration of SCP. The protective effect of 0.4 mg/ml and 0.8 mg/ml SCP on spermatozoa membrane was obviously surpass to positive medicine group. These findings indicate that SCP could be potentially useful for the treatment of oxidative stress damage in spermatozoa.