首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Assessment of genotoxic effect of benzo(a)pyrene in endotracheally treated rat using the comet assay.
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Assessment of genotoxic effect of benzo(a)pyrene in endotracheally treated rat using the comet assay.

机译:使用彗星试验评估气管内处理的大鼠中苯并(a)re的遗传毒性作用。

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Although benzo[a]pyrene (B[a]P) is a well-known genotoxic agent, little is known about the extent of DNA effects induced by B[a]P in rat tissues after pulmonary exposure. The alkaline single-cell gel electrophoresis (comet assay) was used to measure DNA single-strand breaks in alveolar macrophages, lung cells, peripheral lymphocytes and hepatocytes of OFA Sprague-Dawley rats exposed to a single dose of B[a]P by endotracheal administration.Statistically significant damage was observed in all organs tested after 3, 24 and 48h of pulmonary exposure to 3mg of B[a]P per animal, with a time-dependent relationship. The maximum damage was observed in the four cell types 24h after exposure. The higher level of damage was observed both in lung cells and peripheral lymphocytes; in alveolar macrophages and hepatocytes the level of damage was increased, but at a lower level than in the two other cell types. Furthermore, B[a]P demonstrated a clear dose-related genotoxic activity in the lung cells when tested at doses of 0.75, 1.5 and 3mg.The current study shows that B[a]P caused DNA single-strand breaks in the respiratory tract of endotracheally treated OFA Sprague-Dawley rats. The study also suggests that pulmonary exposure to B[a]P can induce a high level of DNA damage in peripheral lymphocytes. The clear relationship between lung exposure to B[a]P and consequences observed in lymphocytes suggests that the comet assay in peripheral lymphocytes can be used as a sensitive marker in human monitoring studies.
机译:尽管苯并[a] re(B [a] P)是众所周知的遗传毒性剂,但对B [a] P在肺部暴露后在大鼠组织中引起的DNA效应程度了解甚少。碱性单细胞凝胶电泳(彗星测定)用于测量气管内暴露于单剂量B [a] P的OFA Sprague-Dawley大鼠的肺泡巨噬细胞,肺细胞,外周淋巴细胞和肝细胞中的DNA单链断裂每只动物在3、24和48h肺暴露于3mg B [a] P后,在所有测试的器官中均观察到统计学上显着的损伤,并具有时间依赖性。暴露后24小时,在四种细胞类型中观察到最大损伤。在肺细胞和外周淋巴细胞中均观察到较高的损伤水平。在肺泡巨噬细胞和肝细胞中,损伤的程度有所增加,但低于其他两种细胞类型。此外,当以0.75、1.5和3mg的剂量进行测试时,B [a] P在肺细胞中显示出明显的剂量相关的遗传毒性活性。当前研究表明,B [a] P导致呼吸道DNA单链断裂。经气管内治疗的OFA Sprague-Dawley大鼠的数量。这项研究还表明,肺部暴露于B [a] P会在外周淋巴细胞中引起高水平的DNA损伤。肺部接触B [a] P的暴露与淋巴细胞中观察到的后果之间的明确关系表明,外周淋巴细胞中的彗星试验可以用作人类监测研究中的敏感标记。

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