Chemical Modification of Catalytically Essential Functional Groups of NAD-Dependent ydrogenase from Ralstonia eutropha H16

摘要

Amino acid residues His and Cys of the NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Ralstonia eutropha H16 were chemically modified with specific reagents.The modification of His residues of the nonactivatied hydrogenase resulted in decrease in both hydrogenase and diaphorase activities of the enzyme.Activation of NADH hydrogenase under anaerobic conditions additionally modified a His residue (or residues)significant only for the hydrogenase activity.The rate of decrease in the diaphorease activity was unchanged.The modification of thiol groups of the nonactivated enzyme did not affect the hydrogenase activity.The effect of thiol-modifying agents on the activated hydrogenase was accompanied by inactivation of both diaphorase and hydrogenase activities.The modification degree and changes in the corresponding catalytic activities depended on conditions of the enzyme activation.Data on the modification of cysteine and histidine residues of the hydrogenase suggested that the enzyme activation should be associated with significant conformational canges in the protein globule.