首页> 外文学位 >Elementary Mode Analysis of Ralstonia Eutropha H16 Metabolism for the Production of Useful Metabolites.
【24h】

Elementary Mode Analysis of Ralstonia Eutropha H16 Metabolism for the Production of Useful Metabolites.

机译:Ralstonia Eutropha H16代谢用于生产有用代谢物的基本模式分析。

获取原文
获取原文并翻译 | 示例

摘要

Ralstonia eutropha H16 is a Gram-negative, facultative chemolithoautotrophic bacterium with the capability to synthesize many useful metabolites. One of the keys to the organism's lifestyle is its ability to use—alternatively or concomitantly—both organic compounds and molecular H2 as sources of energy. It can fix CO 2 via the Calvin-Benson-Bassham (CBB) cycle and produces several useful metabolites like Poly(3-hydroxybutyric acid), isobutanol, 2,3-butanediol and ethanol. To quantitatively evaluate the capabilities of the metabolism we have set up models of the central metabolism that is based on known pathways of the organism. The lithoautotrophic metabolic model consists of 29 reversible reactions, 33 irreversible reactions, 59 internal metabolites and 11 external metabolites exchanged through the cell membrane. The heterotrophic model with fructose as a substrate has 31 reversible reactions, 47 irreversible reactions, 66 internal metabolites and 13 external metabolites. Elementary Mode Analysis identified 759 modes during lithoautotrophic growth and 135074 modes during heterotrophic growth. We have used the results from this analysis to predict key genetic alterations in the metabolism that would direct the metabolic flux towards the production of ethanol, isobutanol or polyhydroxybutanoate.
机译:Ralstonia eutropha H16是革兰氏阴性兼性化石自养细菌,具有合成许多有用代谢产物的能力。生物体生活方式的关键之一是其可替代地或同时地使用有机化合物和分子H2作为能源的能力。它可以通过Calvin-Benson-Bassham(CBB)循环固定CO 2并产生几种有用的代谢物,例如聚(3-羟基丁酸),异丁醇,2,3-丁二醇和乙醇。为了定量评估新陈代谢的能力,我们建立了基于生物体已知途径的中央新陈代谢模型。岩石自养代谢模型由通过细胞膜交换的29个可逆反应,33个不可逆反应,59个内部代谢物和11个外部代谢物组成。以果糖为底物的异养模型具有31个可逆反应,47个不可逆反应,66个内部代谢物和13个外部代谢物。基本模式分析确定了自养营养生长过程中的759种模式和异养生长过程中的135074种模式。我们已经使用了该分析的结果来预测代谢中的关键遗传改变,这些改变将使代谢通量转向乙醇,异丁醇或聚羟基丁酸酯的生产。

著录项

  • 作者

    Lopez, Gilsinia.;

  • 作者单位

    University of Minnesota.;

  • 授予单位 University of Minnesota.;
  • 学科 Biology Microbiology.;Computer Science.;Engineering Chemical.
  • 学位 M.S.
  • 年度 2013
  • 页码 139 p.
  • 总页数 139
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号