P NMR Spectroscopy Revealed Adenylate kinase-1 ike Activity and Phosphotransferase-like Activity from F1-ATPase of Escherichia coli

摘要

Adenylate kinase-like activity and phosphotransferase-like activity from F1-ATPase of Escherichia coli was revealed by ~(31)P NMR spectroscopy. Incubation of Fi-ATPase with ADP in the presence of Mg~(2+) shows the appearance of ~(31)P resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of Fi-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of Fi-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from Fi-ATPase of Escherichia coli. ~(31)P NMR could be a valuable tool for the investigation of phosphorous related enzyme.