Screening and Selectionof GPCR-Expressing Cell Lines:A Rapid Method to Screen and Pick Cells with Optimal Expression Levels

摘要

To meet the demands of high-throughput screening and selectivity profiling, robust cloning and expression systems are needed. Establishing cell lines expressing GPCRs of interest provide great advantage in charac-terization studies of receptor-mediated signaling and screening campaigns of novel therapeutic drug candidates. Identification and isolation of GPCR clones provide a source of GPCR proteins for a variety of applications including cell-based functional assays for hard-to-express GPCRs.The ClonePix 2 System is a platform enabling the identification and isolation of GPCR clones in an accurate and rapid manner. Utilizing white light and fluorescent based imaging in situ, the ClonePix 2 System has both the sensitivity and specificity to quantitatively detect various expression levels of cell surface protein, thus providing a solution to screening both endogenous and overexpressed levels of GPCR protein.The ability to screen and rapidly pick large pools positive expressing cells improve the throughput required for cell line development. Thus the ClonePix 2 System provides a unique solution to shorten time lines involved in target validation and development of GPCR expressing cell lines.