IL-7 IL-7 expression reporter cell lines and methods for screening therapeutic agents for immunodeficiency diseases using the cell lines

摘要

The present invention is IL-7 expression of the reporter cell line and as it related to immunodeficiency disease treatment agent screening method, the present inventors have Cas9 gene - using a targeting techniques, the reporter to observe the intrinsic IL-7 expression of the transcriptional level in a human cell line we developed the system. In addition, the present inventors have IL-7 pilot for the compound to induce the up-regulation in the HTS format were excavating was carried out screening, IL-7 compound with up to control gene transcription. Thus, IL-7 and IL-7 reporter system reporter cell line of the invention can be very useful for screening for IL-7-modulating compound in HTS format.;