Detection of GI and GII noroviruses in drinking water and vegetables using filtration and real-time RT-PCR

摘要

The purpose of the study was to provide a rapid and sensitive method for detecting NoV GI and NoV GII in drinking water and vegetables. The method is based on viral concentration by microporous membrane adsorption method before RNA extraction and real-time RT-PCR amplification. Then water and vegetable samples which artificially contaminated with NoV GI and GII stool samples were used to determine the mean virus recoveries and the method sensitivity. The method showed the detection limit of NoV GI was 4.13 x 10(2) copies/500 mL for drinking water and 4.13 x 10(3) copies/15 g for lettuce and coriander. The detection limit of NoV GII was 2.94 x 10(1) copies/500 mL for distilled water, 2.94 x 10(2) copies/500 mL for Mountain spring water and mineral water, and 2.94 x 10(3) copies/15 g for lettuce and coriander. The method described provides a valuable tool for monitoring the potential public health risks associated with noroviruses contamination in drinking water and vegetables.