Simultaneous Spectrophotometric Determination Of Coumaric Acid, Rutin And Quercetin In Biological Fluids By Means Of Some Multivariate Calibration Methods

摘要

Due to biological activities of Coumaric acid (COR), and Rutin (RUT) and Quercetin (QUR) could be a decent lead compound for a new medication development. Thus, the simultaneous determination of three analytes with similarstructure is required. Additionally, investigates on the simultaneous analysis of COR, RUT, and QUR in biological specimens are rare by spectrometry [l-5].The use of multivariate calibration for the simultaneous determination of COR, RUT and QUR is depicted. Along these lines, a simple, quick and inexpensive method for the simultaneous determination of these anti-oxidants was explored and created with the support of chemometrics methods comparing the principal component regression (PCR) and partial least squares (PLS) models[6-9]. Despite the fact that the components demonstrate an important degree of spectral overlap, they have been simultaneously determined with high accuracy and precision, rapidly and with no need of nonaqueous solvents for dissolving the specimens. It is conceivable to acquire a model adjusted in accordance with the concentration values of the mixtures used in the calibration range[8]. UV-spectra were gathered in the 220-350 nm range.The standard curves were found to be linear over the concentration ranges of 0.1-5.00 μg/mL for COR, RUT and QUR. The PRESS for COR, RUT and QUR with PLS were 2.9915, 0.7292 and, 2.5817 that, those NPC were 4, 5, and 3 but in PCR model the PRESS were 2.7902, 2.4827, 4.057 and NPC were 6, 8, 5, respectively. This technique permits the simultaneous determination of anti-oxidant in synthetic and genuine samples.