PLANT REGENERATION FROM EMBRYOGENIC SUSPENSION CULTURES OF CAMELLIA JAPONICA

摘要

Two methods (I and II) for somatic embryo production from embryogenic suspension cultures of Camellia japonica are presented. Method I, embryogenic suspension cultures, was established from suspension cultures initiated from leaf-derived callus. These cultures were maintained by reducing agitation and increasing subculture interval. Induction of somatic embryogenesis was achieved in MS28 medium, 6, 12, 24, and 36 mo. after culture establishment. Embryo production decreased after 1 yr of culture. Method II, suspensions of single embryogenic cells and proembryos, was obtained from leaves cultured in liquid MS13 medium 6 wk after culture initiation. Embryo production was 23 embryos/ml. Germination of cell suspension-derived embryos on MS56 medium was 16.7% (+/-4.2%) for method I, and 35.4% (+/-5.1%) for method II. The embryos germinated into plantlets with 0 to 7 axillary shoots.