Cost-ettective parentage verification with 17-plex PCR for goats and 19-plex PCR for sheep

摘要

Description: The use of microsatellite markers for parentage verification is well established. SNPs are in discussion too but not yet an alternative for systematic use. Microsatellites can be multiplexed into a single PCR, creating an easy and cost-effective assay. However, multiplex systems containing a large number of microsatellites are not generally available for small ruminants. For parentage testing in goat, Luikart et al. developed a two-set system, each containing 11 microsatellite markers. Each set is amplified in three multiplex groups and run in one gel lane (on an ABI 377 DNA sequencer; ABI). There is also a parentage testing system for goats that includes two sets of 6 and 8 microsatellites respectively and another parentage testing system for sheep that includes two sets of 7 and 8 microsatellites respectively.