Development of a rapid PCR-Nucleic Acid Lateral Flow Immunoassay (PCR- NALFIA) based on rDNA IGS sequence analysis for the detection of Macrophomina phaseolina in soil

摘要

The 'Nucleic Acid Lateral Flow Immunoassay' (NALFIA) using a generic 'Lateral Flow Device' (LFD), combined with PCR employing labelled primers (PCR-NALFIA), enables to circumvent the use of electrophoresis, making the diagnostic procedure more rapid and easier. If the specific amplicon is present in the sample, a coloured band, with an intensity proportional to the amplicon concentration, will develop on the LFD strip in addition to the control band.