DNA metabarcoding to assess indoor fungal communities: Electrostatic dust collectors and Illumina sequencing

摘要

DNA metabarcoding is increasingly being used to characterize the microbiological composition of both the indoor and outdoor environments of dwellings. Our study aimed to evaluate metabarcoding and bioinformatic analysis resulting from calibrated samples and samples collected by an electrostatic dust collector (EDC) in dwellings with no moisture problems. Thus, the fungal communities of 14 dwellings (eastern France, Franche-Comte region) were analyzed by Illumina MiSeq technology after amplification of the ITS2 region.