Morphological and genetic characterization, optimization of fermentation conditions and antioxidant activity of Xiaojin Tricholoma matsutake

摘要

In the present study, morphological and genetic characterization of the Tricholoma matsutake was identified from the fruiting bodies of Xiaojin Tricholoma matsutake in southwestern China. The morphological character of Xiaojin sample is coincident with the character of Tricholoma matsutake genus and a phylogenetic tree was also generated based on the sequences of the internal transcribed spacer (ITS) region of related fungi. The analysis of ITS sequence showed that fungus was clustered together withTricholoma matsutake (AF367418.1) in the phylogenetic tree, which indicated that fungus was a kind of fungi belonging to Subgenus Tricholoma. Meanwhile, the improved cPDA medium was identified as the most suitable medium components for solid culture. Orthogonal design was used to test the relative importance of various factors on liquid fermentation. Among the variables, rotary speed found to be the most significant variables. From further optimization studies, the optimized values of the variables forproduction were as follows: pH 5.5, temperature 20°C, speed 200 r/min, inoculation volume 20% and vaccination age 5 d. In the study, the highest production of 33.365 g/1 mycelium dry weight was obtained with the production of 0.4958248 g/1 total sugar.The polysaccharide isolated from the mycelium of Xiaojin Tricholoma matsutake showed relative strong antioxidant activity using DPPH" radical scavenging method. The antioxidant activity for DPPH" radical and the concentration of the T-1 was positively correlated.