Amniotic fluid, an effective factor for umbilical cord blood hematopoietic stem cells in cell culture: An approach for bone marrow transplantation

摘要

Bone marrow transplantation is a treatment used for hematologic and non-hematologic disorders. A theory suggests that proliferation of cells in non-body condition helps to increase the efficiency of bone marrow transplant. There are different ways for proliferation of stem cells, in which, most studies have focused on stem cell culture in body-like conditions. The use of amniotic fluid as a rich resource of growth factors is developing in repair of tissues cornea. With regards to this condition, we discuss about the influence of amniotic fluid in proliferation and implantation of blood stem cells. The aim of this study was investigation of human amnion fluid (HAF) in support of growth and proliferation of umbilical cord in order to transplant and long period erythropoiesis. First, separating of CD-34(+) stem cells by MACS was performed and check in 5% and 8% concentration of amniotic fluid (AF) in comprise with FBS10% in culture environment. After 7, 14 days cell count, and checking gene expression level of cyclinD1, BCL2, CXCR4, SDF1 by real-time PCR. The result show that BCL2, CXCR4 and cyclinD1 gene expression level were increased in cells that are growth in 5% AF with 5% FBS than other groups. After statistical analysis, proliferation of umbilical cord blood stem cells in 5% AF with 5% FBS was more than 8% AF with 2% FBS and 10% FBS. Therefore, HAF can play an effective role in increasing hematopoietic stem cells in cell culture before bone marrow transplant.