Whole-genome multilocus sequence typing of closely related broiler chicken meat origin Campylobacter jejuni ST-5 isolates

摘要

Broiler chicken meat is considered as the main source of foodborne Campylobacter infection. Campylobacter jejuni multilocus sequence type ST-5 has been previously identified as predominant among Latvian and Lithuanian broiler chicken meat products and is often isolated from Lithuanian patients with Campylobacter enteric infection. The market share of Lithuanian and Latvian origin fresh broiler chicken meat sold at Estonian retail is estimated close to 50%. In this study, we used the whole-genome sequences of Latvian and Lithuanian origin C. jejuni ST-5 broiler chicken meat isolates obtained from Estonian retail. Applied genome-by-genome whole-genome MLST revealed high similarity of investigated isolates with 1589 shared alleles, of which 218 loci were polymorphic. Latvian and Lithuanian isolates had 199 to 205 intergroup differences in shared alleles, whereas intragroup isolates differed by 27 to 31 alleles. Allele differences divided the isolates into 2 distinct groups based on the country of origin, which was supported by sequence-based phylogenetic tree. The resulting geographic separation of the isolates found in this study should be carefully interpreted taking into account the small sample size.