Detection of Yersinia enterocolitica species in pig tonsils and raw pork meat by the real-time PCR and culture methods

摘要

The aim of the present study was to establish a rapid and accurate real-time PCR method to detect pathogenic Yersinia enterocolitica in pork. Yersinia enterocolitica is considered to be a crucial zoonosis, which can provoke diseases both in humans and animals. The classical culture methods designated to detect Y enterocolitica species in food matrices are often very time-consuming. The chromosomal locus_tag CH49_3099 gene, that appears in pathogenic Y enterocolitica strains, was applied as DNA target for the 5' nuclease PCR protocol. The probe was labelled at the 5' end with the fluorescent reporter dye (FAM) and at the 3' end with the quencher dye (TAMRA). The real-time PCR cycling parameters included 41 cycles. A Ct value which reached a value higher than 40 constituted a negative result.