Development of Potato virus Y (PVY) resistant pepper (Capsicum annuum L.) lines using marker-assisted selection (MAS)

摘要

Several alleles at the pvr2 locus are known to control recessive resistance to Potato virus Y (PVY) in pepper. In this study, PVY resistant F-2 pepper lines were developed from local germplasm carrying recessive genes (pvr2(1) and pvr2(2)) using marker-assisted selection (MAS). The F-1 and F-2 generations were assessed for the presence of resistant/susceptible alleles (pvr2(+)/pvr(1)/pvr2(2)) at the pvr2-elF4E locus using the tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) procedure. Our results show that ARMS-PCR can be used to successfully screen pepper genotypes for alleles that confer PVY resistance thereby contributing to the improvement of pepper production.