Molecular marker-assisted selection for development of yellow seeded Pol cms restorer lines in Brassica napus L.

摘要

The primary objective of this work was to transfer the partial-dominant yellow-seeded gene derived from a stable and pure yellow-seeded Brassica napus DH line No.2127-17 to Hui5148-2 to breed homozygous Polima cms restorer lines. To achieve this goal, the procedure of molecular marker-assisted selection (MAS) combined with phenotypic selection was performed in a modified recurent backcrossing program. One dominant SCAR marker SCS1130 and one co-dominant CAPS marker SCA1 which closely flanked to the yellow-seeded gene and identified in our previous study were carried out in each segregating progenies to select the individuals carrying yellow-seeded gene. Simultaneously, a total of 88 RAPD primers and 60 AFLP primer pairs assays were used to analyze the background with a two-step selected approach, to identify the yellow-seeded plants genetically closer to the recurrent parent Hui5148-2 in BC1F1 and BC2F1, respectively. BC2F1 plants were finally self-pollinated to produce BC2F2 As a result, 5 superior introgression lines were identified for homozygous yellow-seeded restorer lines using SCA1 marker and further confirmed by phenotypic assessment in BC2F2:3. Background analysis subsequently demonstrated the 5 lines with 0.0157-0.0364 relative genetics distance to Hui5148-2. Further quality analysis and field trails consequently showed that they resulted in enhancing oil content and improving quality with desirable agronomic traits, which would offer the elite restorers in yellow-seeded hybrids breeding project.