Dynamic Changes in MMPl and TIMP1 in the Antifibrotic Process of Dahuang Zhechong Pill in Rats with Liver Fibrosis

摘要

On the basis of carbon tetrachloride(CCl4)-induced liver fibrosis in rats,this study aims to investigate the dynamic changes in matrix metalloproteinase 1(MMP1)and the tissue inhibitor of metalloproteinase 1(TIMP1)in the antifibrotic process of Dahuang Zhechong Pill(DHZCP).A total of 50 male Sprague Dawley rats,aged 8 weeks,were randomly divided into 3 groups: the control group,the model group(the group treated with CCl4),and the treatment group(the group treated with CCl4 and DHZCP).Rats were sacrificed at Weeks 4 and 8.Liver tissues were separated for RNA sequencing and bioinformatics analysis.Real-time PCR,Western blot analysis,and histological staining were conducted to confirm the gene expression and pathological change in liver tissues.Compared with control group,rats in model group showed poor mental state and slow weight gain.The liver tissues of the rats in the model group exhibited a damaged hepatic lobule structure,fibrous connective tissue hyperplasia,and inflammatory cell infiltration among the hyperplastic tissues.DHZCP could significantly improve the appearance of rats and alleviate CC1-induced fibrosis.Compared to model group,798 differentially expressed mRNAs were found in the treatment group,of which 120 were up-regulated and 678 were down-regulated.Differentially expressed mRNAs between the CCl4-induced group and the DHZCP-treated group were mainly focused on the following KEGG pathways: focl adhesion,phagosome,tight junction,and ECM-receptor interactions.Relative to those in the control group,MMPl was downregulated,whereas,TIMPl and Col1A1 were upregulated in the CCl4-induced group at Weeks 4 and 8.DHZCP could reverse MMPl,TIMPl,and Col1A1 expression.DHZCP protects against liver injury and exerts an antifibrotic effect on liver fibrosis induced by CCl4 in rats.Its mechanism may be related to the upregulation of MMPl,downregulation of TIMPl,and promotion of collagen degradation.