Development and validation of an RP-HPLC method for simultaneous determination of sorbic acid, benzoic acid, and natamycin in domestic yogurt in Jordan

摘要

A method for the simultaneous determination and analysis of sorbic acid, benzoic acid, and natamycin in yogurt products, based on the use of the high-performance liquid chromatography with a UV detector (HPLC-UV) has been developed. This method excludes the employment of complex procedures, time-consuming and labor-intensive pre-treatment processes. Separation of the sorbic acid, benzoic acid, and natamycin with higher selectivity and sensitivity, and within five minutes was performed by a C8 150 mm × 4.6 mm × 3 μm column (Hypersil BDS) at 20°C. The mobile phase was a mixture of trifluoroacetic acid 0.1% in water containing 100 mM sodium acetate, trifluoroacetic acid 0.1% in acetonitrile, and trifluoroacetic acid 0.1% in tetrahydrofuran (65:25:10 (%, v/v)). Using the mobile phase as an extraction mixture gave recoveries in the range of 83.0-110.2% at spike levels of 2.5-80.0 μg/kg with relative standard deviations (RSDs) lower than 10%. The intra-day precision and inter-day precision results were in the range of 5.3-6.7% and 7.6-9.2%. Additionally, the limits of detection (LOD) were 0.24, 0.39, and 0.61 mg/L and the limits of quantification (LOQ) were 0.80, 1.3, and 2.0 mg/L for sorbic acid, benzoic acid, and natamycin, respectively.