Enhancing the Production of Rhodobacter sphaeroides-Derived Physiologically Active Substances Using Carbonic Anhydrase-Immobilized Electrospun Nanofibers

摘要

In order to achieve continuous biocatalytic hydration of CO2, carbonic anhydrase (CA) derived from Rhodobacter sphaeroides was immobilized on electrospun polystyrene/poly(styrene-co-maleic anhydride) (PS/PSMA) nanofibers as cross-linked enzyme aggregates (CLEA). The CA-CLEA maintained more than 94.7% of its initial activity during 60 days of storage perio d at 4 °C and also retained more than 45.0% activity after 60 reuses. The capability of CA-CLEA to hydrolyze CO2 was verified in terms of CaCO, precipitation. The immobilized CA induced enhanced cell growth of R. sphaeroides, which then increased the production of R. sphaeroides-derived organic substances including carotenoid, bacterio-chlorophyll, porphyrin, and coenzyme Q10. Further exploitation of such nanofiber-catalyst complexes that allows environment friendly use of CO2 is expected in a variety of research fields.