Preparation and reactivity fo a novel disaccharide,glucosyl 1,5-anhydro-D-fructose (1,5-anhydro-3-O-alpha-glucopyranosyl-D-fructose)

摘要

A novel disaccharide,glucosyl 1,5-anhydro-D-fructose (1,5-anhydro-3-O-alpha-glucopyranosyl-D-fructose,GAF)was enzymatically prepared from 1,5-anhydro-D-fructose (1,5-AF)and cyclomaltoheptasoe (beta-cyclodextrin).Cyclodextrin glucanotransferase transferred viaious sizes of maltooligosaccharide to 1,5-AF.Glucoamylase digested the maltooligosyl chain of the products to a glucosyl residue giving a final product,GAF.An NMR analysis of GAFG elucidated that the glucose residue was linked to C-3 of the 1,5-AF residue with an ether linkage.Reactivity on the aminocarbonyl reaction f GAF with bovine serum albumin was lower than that of 1,5-AF but was higher tha that of glucose.