Electrochemical investigation on ionizable levofloxacin transfer reaction across liquid/liquid interfaces and potential applications to milk analysis

摘要

The transfer process of an ionizable fluoroquinolone antibacterial drug, levofloxacin (LVX), at a polarized interface between two immiscible electrolytes solutions (ITIES) has been investigated for the first time using voltammetric techniques. The charged nature of ionizable LVX species at various aqueous pH solutions with controlled ionic strength were characterized and further used for establishing an ionic partition diagram of LVX. The results led us to predict the dominant charge species with respect to the pH of aqueous solutions and LVX lipophilicity in addition to the thermodynamics information of the LVX transfer process. A voltammetric sensor featuring a microhole supported water/organic gel interface, based on measuring current changes associated with the protonated LVX (HLVX+) transfer reaction across the interface, was also developed. Our HLVX+sensitive sensor in conjunction with a differential pulse stripping voltammetry (DPSV) technique was employed for the quantification of HLVX+in buffer solution and further for food sample via spiking HLVX+in a commercially available milk solution. Our sensing results were finally validated with those using high-performance liquid chromatography (HPLC) analyses.