...
首页> 外文期刊>Journal of Agricultural and Food Chemistry >Determination of the Ergot Alkaloid Ergovaline in Tall Fescue Seed and Straw Using a QuEChERS Extraction Method with High-Performance Liquid Chromatography-Fluorescence Detection
【24h】

Determination of the Ergot Alkaloid Ergovaline in Tall Fescue Seed and Straw Using a QuEChERS Extraction Method with High-Performance Liquid Chromatography-Fluorescence Detection

机译:高效液相色谱-荧光检测QuEChERS萃取法测定高羊茅种子和稻草中麦角生物碱麦角灵碱

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

Ergovaline is an ergot alkaloid produced by the symbiotic endophyte Epichloe coenophiala, which can colonize varieties of the cool-season grass tall fescue (Festuca arundinacea). It is the principle toxicant responsible for the vasoconstrictive and reproductive sequelae seen in "fescue toxicosis" in livestock which consume forage exceeding the threshold of toxicity established for this compound. A new method for extraction of ergovaline from tall fescue seed and straw was optimized and validated, on the basis of the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, with high-performance liquid chromatography-fluorescence detection. Fourteen extraction solvents were tested; 2.1 mM ammonium carbonate/acetonitrile (50/50, v/v) had the highest and most consistent recovery (91-101%). Linearity, limit of detection, limit of quantitation, accuracy,and intra- and interday precisions for tall fescue seed and straw were 100-3500 mu g/kg, 37 and 30 mu g/kg, 100 mu g/kg, 98%, 3.0 and 1.6%, and 3.8 and 1.0%, respectively. When the currently used solid-phase extraction (SPE) and QuEChERS methods were applied to 17 tall fescue straw samples, there was good agreement (correlation coefficient 0.9978). The QuEChERS method achieved the goals of eliminating chlorinated solvents and developing a fast, efficient, reliable method for quantitating ergovaline in tall fescue forage that can be applied in a high-throughput food safety laboratory.
机译:麦角碱是一种由共生内生真菌Epichloe coenophiala产生的麦角生物碱,可定居在凉季草高羊茅(Festuca arundinacea)的变种中。它是造成牲畜“羊茅中毒”中血管收缩和生殖后遗症的主要有毒物质,牲畜的饲料消耗超过该化合物确定的毒性阈值。在QuEChERS(快速,简便,廉价,有效,坚固耐用和安全)方法的基础上,采用高效液相色谱-荧光检测法优化和验证了从高羊茅种子和秸秆中提取麦角新碱的新方法。测试了十四种萃取溶剂; 2.1 mM碳酸铵/乙腈(50/50,v / v)具有最高和最一致的回收率(91-101%)。高羊茅种子和稻草的线性,检测限,定量限,准确度以及日内和日间精度分别为100-3500μg / kg,37和30μg / kg,100μg / kg,98%,分别为3.0%和1.6%,以及3.8%和1.0%。当将当前使用的固相萃取(SPE)和QuEChERS方法应用于17个高羊茅秸秆样品时,有很好的一致性(相关系数0.9978)。 QuEChERS方法实现了消除氯化溶剂的目标,并开发了一种快速,高效,可靠的定量高羊茅饲料中麦角灵碱的方法,该方法可用于高通量食品安全实验室。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号