Application of High-Speed Countercurrent Chromatography for the Isolation of Sulforaphane from Broccoli Seed Meal

摘要

In order to produce large amounts of pure sulforaphane tor researcn purposes,a novel method using high-speed countercurrent chromatography(HSCCC)was developed.Without any initial cleanup steps,sulforaphane was successfully purified from the ethyl acetate extract of the broccoli seed meal by HSCCC.The separation was performed with two-phase solvent systems:n-hexane/ethyl acetate/methanol/water(1:5:1:5,v/v/v/v).From 850 mg of the ethyl acetate extract,186 mg of sulforaphane was isolated with the solvent system.The purified compound was over 97% purity as determined by HPLC analysis,and the chemical structure was confirmed by MS and ~1H and ~(13)C NMR.