Extraction of sulforaphane from broccoli seed extract containing glucoraphanin, comprises hydrolyzing the extract, and extracting carbohydrates and lipids in hydrolyzed extract, by water containing sulforaphane

摘要

The extraction of sulforaphane from broccoli seed extract containing glucoraphanin, comprises hydrolyzing the extract, and extracting carbohydrates and lipids in the hydrolyzed extract, by a water (1:1 weight ratio) containing sulforaphane. The extraction of carbohydrates and lipids is carried out using an ion exchange resin and a solvent in which the carbohydrates and/or the lipids are not soluble. The extraction of carbohydrates and lipids is carried out by water before evaporation of the higher phase. The evaporation is carried out using a rotary evaporator at 30[deg]C. The extraction of sulforaphane from broccoli seed extract containing glucoraphanin, comprises hydrolyzing the extract, and extracting carbohydrates and lipids in the hydrolyzed extract, by a water (1:1 weight ratio) containing sulforaphane. The extraction of carbohydrates and lipids is carried out using an ion exchange resin and a solvent in which the carbohydrates and/or the lipids are not soluble. The extraction of carbohydrates and lipids is carried out by water before evaporation of the higher phase. The evaporation is carried out using a rotary evaporator at 30[deg]C. The first extraction process comprises adding an ethylacetate (1:1 volume ratio) to the hydrolyzed extract, centrifuging the basic ethylacetate solution to obtain a first supernatant solution and decanting the supernatant solution to obtain a first higher and lower aqueous phase. The second extraction process comprises mixing a base and the lower aqueous phase, adding the ethylacetate (1:1 volume ratio) to the mixture, centrifuging the ethylacetate mixture to obtain a second base and a second supernatant solution, decanting the supernatant solution to obtain second higher and lower aqueous phase, and extracting higher phases to collect the lower phases. The third extraction process comprises mixing the second base and the second lower aqueous phase, adding ethylacetate to the mixture, centrifuging the ethylacetate mixture to obtain a third base and a third supernatant solution and decanting the supernatant solution to obtain a third higher and lower aqueous phase. Freeze-drying and matrix encapsulation of the sulforaphane are obtained from the extraction of carbohydrates and lipids by water. The matrix encapsulation is carried out using gum arabic. Mixing the gum arabic with the sulforaphane before freeze-drying. The mixture of sulforaphane and gum arabic comprises 70% of gum arabic.