Study on Polymer Formation and Film-forming Properties Under Catalysis of Transglutaminase Between Casein and Keratin

摘要

Different mass ratio of casein and keratin were used to produce heteropolymer under the treatment of transglutaminase. The molecular weight of the polymer was investigated by SDS-PAGE. Films mechanical and water vapor barrier properties of the blended film were also studied. Film morphology was observed by using scanning electron microscopy. The crosslinking between casein and keratin proved by electrophoresis and the molecular weight of the new polymer was presumed about 50-70 kDa. The casein/keratin blended films showed an increase in elongation and water vapor permeability with transglutaminase treatment. The synergistic effect can be seen from the elongation value of the transglutamiase treatment casein/keratin blended filmed at the mass ratio of 4:1. The value of the tensile strength of the blended films decreased significantly with the increasing content of keratin.