Rapid preparation of mycobacterium N-glycolyl lipid i and lipid II derivatives: A biocatalytic approach

摘要

Breaking down barriers: A rapid, inexpensive preparation of the structurally complex mycobacterial N-glycolyl LipidI, LipidII, and their analogues from a range of different synthetic N-glycolyl and N-glycinyl Park's nucleotides is described (see scheme). The biotransformations were catalyzed by a readily available biocatalyst obtained from a bacterial cell-free membrane fraction. The unnatural N-glycinyl LipidII was found to be a substrate of Mycobacterium tuberculosis (Mtb) transglycosylase, PonA, and N-glycolyl LipidI was a weak inhibitor against PonA